Human Hedgehog Interacting Protein (HHIP) ELISA Kit
The Human Hedgehog Interacting Protein (HHIP) ELISA kit is a highly reliable and accurate tool for detecting levels of HHIP in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides researchers with precise and reproducible results, making it an essential component for a variety of research applications.HHIP is a critical protein that interacts with the Hedgehog signaling pathway, which plays a crucial role in embryonic development and tissue regeneration.
Dysregulation of HHIP has been linked to various diseases, including cancer and developmental disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.By using the Human Hedgehog Interacting Protein (HHIP) ELISA Kit, researchers can gain valuable insights into the role of HHIP in disease progression and development, ultimately advancing our understanding of the underlying mechanisms and potential treatment options.
Product Name:
Human Hedgehog Interacting Protein (HHIP) ELISA Kit
Product Code:
HUDL01333
Size:
96 Assays
Target:
HHIP
Synonyms:
HIP
Detection Method:
Competition
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
