The Human Heat Shock Protein Beta 8 (Hspb8) ELISA Kit is a powerful tool for the accurate measurement of Hspb8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Hspb8 is a key heat shock protein that plays a vital role in protecting cells from stress and maintaining cellular homeostasis. Dysregulation of Hspb8 has been implicated in various diseases, including neurodegenerative disorders and cancer, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
With its reliable performance and ease of use, the Human Heat Shock Protein Beta 8 (Hspb8) ELISA Kit is a valuable tool for researchers looking to explore the role of Hspb8 in health and disease. Get accurate and reliable results with this high-quality ELISA kit from Assay Genie.
Product Name:
Human Heat Shock Protein Beta 8 (HSPb8) ELISA Kit
Product Code:
HUDL01395
Size:
96 Assays
Target:
HSPb8
Synonyms:
CMT2L, DHMN2, E2IG1, H11, HMN2, HSP22, Heat Shock 22kDa Protein 8, Alpha-crystallin C chain, E2-induced gene 1 protein, Small stress protein-like protein HSP22
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
