The Human Heat Shock Protein Beta 7 (HSPB7) ELISA Kit is specially designed for the precise and accurate detection of HSPB7 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research applications.Heat Shock Protein Beta 7 (HSPB7) is a key protein involved in cellular stress response and maintenance of cardiac function. Abnormal levels of HSPB7 have been linked to cardiovascular diseases and cardiac dysfunction, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.
With the Human HSPB7 ELISA Kit, researchers can investigate the role of HSPB7 in various biological processes and diseases, leading to a better understanding of its function and potential implications for human health.Upgrade your research with the Human Heat Shock Protein Beta 7 (HSPB7) ELISA Kit and unravel the mysteries of HSPB7 in human physiology.
Product Name:
Human Heat Shock Protein Beta 7 (HSPb7) ELISA Kit
Product Code:
HUDL01394
Size:
96 Assays
Target:
HSPb7
Synonyms:
HSP-B7, cvHSP, Cardiovascular HSP, Heat Shock 27kDa Protein 7, Cardiovascular heat shock protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-99
89
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
