Human Heat Shock 70kDa Protein 1A (HSPA1A) ELISA Kit
The Human HSPA1A (Heat Shock 70kDa Protein 1A) ELISA Kit is specially designed for the precise measurement of HSPA1A levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides accurate and consistent results, making it a valuable tool for a variety of research applications.HSPA1A, also known as Hsp70, is a heat shock protein that plays a crucial role in cellular stress response and protein folding. It is involved in various cellular processes, including protein homeostasis, cell survival, and immune response modulation.
Dysregulation of HSPA1A has been linked to numerous diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance as a biomarker for understanding disease pathology and potential therapeutic interventions.With its reliable performance and versatility, the Human HSPA1A ELISA Kit is an indispensable asset for researchers studying the role of HSPA1A in health and disease, offering valuable insights into its mechanisms and potential therapeutic applications.
Product Name:
Human Heat Shock 70kDa Protein 1A (HSPA1A) ELISA Kit
Product Code:
HUDL01385
Size:
96 Assays
Target:
HSPA1A
Synonyms:
HSP70-1, HSP-A1A, HSPA1, HSP70-2, HSX70
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
