Human Heat Shock 40kDa Protein 2 (HSPF2) ELISA Kit
The Human Heat Shock 40kDa Protein 2 (HSPF2) ELISA Kit is a powerful tool for the precise measurement of HSPF2 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research purposes.HSPF2 is a key heat shock protein involved in cellular stress response and protein folding, essential for maintaining cell homeostasis and survival under challenging conditions.
Dysregulation of HSPF2 has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its significance as a potential diagnostic and therapeutic target.With the Human Heat Shock 40kDa Protein 2 (HSPF2) ELISA Kit, researchers can explore the role of HSPF2 in disease pathogenesis, identify potential biomarkers, and develop novel treatment strategies, ultimately advancing our understanding of cellular stress responses and disease mechanisms.
Product Name:
Human Heat Shock 40kDa Protein 2 (HSPF2) ELISA Kit
Product Code:
HUDL01398
Size:
96 Assays
Target:
HSPF2
Synonyms:
DNAJC4, MCG18, HSP-F2, DnaJ(Hsp40)Homolog,Subfamily C,Member 4, DnaJ-like protein HSPF2, Multiple endocrine neoplasia type 1 candidate protein number 18
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
