The Human HCG (Chorionic Gonadotropin) ELISA Kit is specifically designed for the quantitative measurement of HCG levels in human serum, plasma, and urine samples. This kit offers high sensitivity and specificity, allowing for accurate and reliable results in various research applications.Chorionic Gonadotropin is a hormone produced during pregnancy that plays a crucial role in supporting the development of the embryo and maintaining a healthy pregnancy.
Elevated levels of HCG can indicate pregnancy or certain medical conditions such as trophoblastic diseases or testicular cancer, making it an important biomarker for both diagnosis and monitoring of these conditions.With its easy-to-use protocol and precise results, the Human HCG ELISA Kit is a valuable tool for researchers and healthcare professionals studying reproductive health, pregnancy complications, and related disorders.
Product Name:
Human HCG (Chorionic Gonadotropin) ELISA Kit
SKU:
HUES01443
Target:
Human HCG (Chorionic Gonadotropin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 mIU/mL
Detection range:
7.81-500 mIU/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HCG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HCG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HCG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HCG. You can calculate the concentration of Human HCG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
93-104
97-113
Average (%)
91
99
104
1:4
Range (%)
94-110
86-101
96-110
Average (%)
100
92
103
1:8
Range (%)
101-114
87-101
94-108
Average (%)
108
92
100
1:16
Range (%)
95-110
87-97
93-105
Average (%)
101
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
92
EDTA plasma (n=5)
95-110
101
Cell culture media (n=5)
87-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.5
77.5
224.0
22.4
69.9
213.8
Standard deviation
1.4
3.5
9.4
1.3
3.8
9.2
C V (%)
5.71
4.52
4.2
5.8
5.44
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HCG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HCG in samples. No significant cross-reactivity or interference between Human HCG and analogues was observed.
