Human HB-EGF Recombinant Protein (RPPB0269)
- SKU:
- RPPB0269
- Product type:
- Recombinant Protein
- Size:
- 50ug
- Species:
- Human
- Target:
- HB-EGF
- Synonyms:
- HBEGF
- DTR
- DTS
- HEGFL
- Source:
- Escherichia Coli
- Uniprot:
- Q99075
Description
| Product Name: | Human HB-EGF Recombinant Protein |
| Product Code: | RPPB0269 |
| Size: | 50µg |
| Species: | Human |
| Target: | HB-EGF |
| Synonyms: | HBEGF, DTR, DTS, HEGFL, HB-EGF, Diphtheria toxin receptor, DT-R, DTSF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM sodium phosphate pH-7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized Human HB-EGF in sterile 18M-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Human HB-EGF Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HB-EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MDLQEADLDL LRVTLSSKPQ ALATPNKEEH GKRKKKGKGL GKKRDPCLRK YKDFCIHGECKYVKELRAPS CICHPGYHGE RCHGLSL |
| Biological Activity: | The ED50 was determined by the ability to induce proliferation of 3T3 cells and is 0.13-0.2ng/ml. This corresponds to an expected specific activity of 7.7 x 106units/mg. |
HB-EGF is an EGF related growth factor which signals via the EGF receptor, and stimulates the proliferation of SMC (smooth muscle cells), fibroblasts, epithelial cells and keratinocytes. HB-EGF is expressed in various cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes and particular tumor cells. HB-EGF’s ability to explicitly bind HPR sulfate proteoglycans is dissimilar from other EGF-like molecules, and might be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells.
HB-EGF Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 87 amino acids and having a molecular mass of 9.9kDa.The HB-EGF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | HBEGF: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. |
| UniProt Protein Details: | Protein type:Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 5q23 Cellular Component: extracellular space; cell surface; integral to plasma membrane; extracellular region; plasma membrane Molecular Function:heparin binding; growth factor activity; epidermal growth factor receptor binding Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; muscle development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; negative regulation of elastin biosynthetic process; pathogenesis; signal transduction; positive regulation of cell growth; regulation of heart contraction; positive regulation of protein kinase B signaling cascade; positive regulation of cell proliferation; innate immune response; positive regulation of cell migration; wound healing, spreading of epidermal cells |
| UniProt Code: | Q99075 |
| NCBI GenInfo Identifier: | 544477 |
| NCBI Gene ID: | 1839 |
| NCBI Accession: | Q99075.1 |
| UniProt Secondary Accession: | Q99075,B2R821, |
| UniProt Related Accession: | Q99075 |
| Molecular Weight: | Calculated MW: 23kDaObserved MW: 23kDa |
| NCBI Full Name: | Proheparin-binding EGF-like growth factor |
| NCBI Synonym Full Names: | heparin-binding EGF-like growth factor |
| NCBI Official Symbol: | HBEGF |
| NCBI Official Synonym Symbols: | DTR; DTS; DTSF; HEGFL |
| NCBI Protein Information: | proheparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) |
| UniProt Protein Name: | Proheparin-binding EGF-like growth factor |
| UniProt Synonym Protein Names: | Diphtheria toxin receptor; DT-R |
| Protein Family: | Proheparin-binding EGF-like growth factor |
| UniProt Gene Name: | HBEGF |
| UniProt Entry Name: | HBEGF_HUMAN |
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