Human GTF2E2 Recombinant Protein (RPPB3647)

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SKU:
RPPB3647
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
GTF2E2
Synonyms:
General transcription factor IIE polypeptide 2 beta 34kDa
Synonyms:
transcription initiation factor IIE subunit beta
Synonyms:
TFIIE-beta
Synonyms:
TF2E2
Source:
Escherichia Coli
Uniprot:
P29084
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Description

Product Name:Human GTF2E2 Recombinant Protein
Product Code:RPPB3647
Size:10µg
Species:Human
Target:GTF2E2
Synonyms:General transcription factor IIE polypeptide 2 beta 34kDa, transcription initiation factor IIE subunit beta, TFIIE-beta, TF2E2, TFIIE-B.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GTF2E2 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMDPSLL RERELFKKRA LSTPVVEKRS ASSESSSSSS KKKKTKVEHG GSSGSKQNSD HSNGSFNLKA LSGSSGYKFG VLAKIVNYMK TRHQRGDTHP LTLDEILDET QHLDIGLKQK QWLMTEALVN NPKIEVIDGK YAFKPKYNVR DKKALLRLLD QHDQRGLGGI LLEDIEEALP NSQKAVKALG DQILFVNRPD KKKILFFNDK SCQFSVDEEF QKLWRSVTVD SMDEEKIEEY LKRQGISSMQ ESGPKKVAPI QRRKKPASQK KRRFKTHNEH LAGVLKDYSD ITSSK

GTF2E2 is vital for transcription initiation by RNA polymerase II in conjunction with other general factors. General Transcription Factor IIE is a heterotetramer composed of two subunits of relatively high molecular mass 57,000 (GTF2E1) and two of 34,000 (GTF2E2). GTF2E2 is necessary in conjunction with GTF2E1 for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIE and TFIIH are essential for promoter clearance by RNA polymerase.

GTF2E2 Human Recombinant produced in E. coli is a single polypeptide chain containing 315 amino acids (1-291) and having a molecular mass of 35.6kDa.GTF2E2 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GTF2E2: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Belongs to the TFIIE beta subunit family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

UniProt Code:P29084
NCBI GenInfo Identifier:135232
NCBI Gene ID:2961
NCBI Accession:P29084.1
UniProt Secondary Accession:P29084,Q9H2B9, D3DSV2,
UniProt Related Accession:P29084
Molecular Weight:33,044 Da
NCBI Full Name:Transcription initiation factor IIE subunit beta
NCBI Synonym Full Names:general transcription factor IIE subunit 2
NCBI Official Symbol:GTF2E2  
NCBI Official Synonym Symbols:FE; TTD6; TF2E2; TFIIE-B  
NCBI Protein Information:transcription initiation factor IIE subunit beta
UniProt Protein Name:Transcription initiation factor IIE subunit beta
UniProt Synonym Protein Names:General transcription factor IIE subunit 2
Protein Family:General transcription factor
UniProt Gene Name:GTF2E2  
UniProt Entry Name:T2EB_HUMAN
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