The Human GT-34 (Gastrin-34) CLIA Kit is a cutting-edge assay designed for the precise measurement of Gastrin-34 levels in human biological samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers highly accurate and consistent results, making it an invaluable tool for various research purposes.Gastrin-34 is a key hormone involved in regulating gastric acid secretion and plays a crucial role in gastric functions. Dysregulation of Gastrin-34 levels has been linked to conditions such as peptic ulcers, gastrinomas, and gastroesophageal reflux disease, highlighting its significance as a potential biomarker for studying and managing these disorders.
Whether investigating the pathophysiology of gastrointestinal diseases or exploring novel therapeutic interventions, the Human GT-34 CLIA Kit offers researchers a reliable and efficient means of quantifying Gastrin-34 levels, paving the way for advancements in the field of gastroenterology.
Product Name:
Human GT-34 (Gastrin 34) CLIA Kit
SKU:
HUES01099
Target:
Human GT-34 (Gastrin 34)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GT-34. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GT-34 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GT-34, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GT-34. You can calculate the concentration of Human GT-34 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
97-113
86-100
Average (%)
90
104
93
1:4
Range (%)
97-112
87-102
93-108
Average (%)
105
94
101
1:8
Range (%)
84-97
85-98
95-109
Average (%)
90
90
103
1:16
Range (%)
85-97
100-115
86-99
Average (%)
91
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
100
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
94-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.37
60.04
228.4
24.87
64.7
249.03
Standard deviation
2.78
6.04
25.7
2.75
6.13
28.36
C V (%)
11.41
10.06
11.25
11.06
9.47
11.39
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GT-34 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GT-34 in samples. No significant cross-reactivity or interference between Human GT-34 and analogues was observed.
