The Human GS (Gelsolin) ELISA Kit is specifically developed for the precise measurement of gelsolin levels in human samples including serum, plasma, and cell culture supernatants. With its impressive sensitivity and specificity, this kit delivers consistent and accurate results, making it an excellent choice for various research purposes.Gelsolin is a key protein known for its role in regulating actin dynamics, cell movement, and apoptosis. Dysregulation of gelsolin has been linked to various diseases such as cancer, inflammatory disorders, and neurodegenerative conditions.
Therefore, the Human GS (Gelsolin) ELISA Kit serves as a valuable tool for studying these diseases and exploring potential therapeutic interventions.Get reliable and insightful data with the Human GS (Gelsolin) ELISA Kit, enabling in-depth research and advancing our understanding of gelsolin's involvement in health and disease.
Product Name:
Human GS (Gelsolin) ELISA Kit
SKU:
HUES02756
Target:
Human GS (Gelsolin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GS. You can calculate the concentration of Human GS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-112
85-98
Average (%)
91
103
91
1:4
Range (%)
92-106
84-96
89-104
Average (%)
98
90
95
1:8
Range (%)
94-106
79-92
86-100
Average (%)
99
85
92
1:16
Range (%)
93-108
79-91
84-96
Average (%)
100
85
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
102
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
19.74
55.47
186.36
20.68
50.26
195.03
Standard deviation
1.12
2.82
8.35
1.21
2.62
10.12
C V (%)
5.67
5.08
4.48
5.85
5.21
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GS in samples. No significant cross-reactivity or interference between Human GS and analogues was observed.
