Human Growth Regulated Oncogene Gamma (GROg) ELISA Kit

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SKU:
HUDL01265
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19876
Sensitivity:
2.7pg/mL
Range:
7.8125-500pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL3,SCYB3,GRO3,GRO-G,MIP2-B,MIP2b,CINC2b,CINC2b,Chemokine(C-X-C-Motif)ligand 3,Macrophage inflammatory protein 2-beta
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human Growth Regulated Oncogene Gamma (GROg) ELISA Kit

The Human GROG (Growth Regulated Oncogene Gamma) ELISA Kit is specially designed for the precise measurement of GROG levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a wide range of research applications.GROG, also known as CXCL3, is a vital growth factor involved in regulating cell proliferation, migration, and inflammation.

It plays a crucial role in various biological processes, including tumorigenesis, wound healing, and immune response. As such, it is a valuable biomarker for studying cancer, inflammatory diseases, and other conditions, as well as for developing potential therapeutic interventions. Order the Human GROG ELISA Kit today and unlock new possibilities in your research endeavors.

Product Name:Human Growth Regulated Oncogene Gamma (GROg) ELISA Kit
Product Code:HUDL01265
Size:96 Assays
Target:GROg
Synonyms:CXCL3, SCYB3, GRO3, GRO-G, MIP2-B, MIP2b, CINC2b, CINC2b, Chemokine(C-X-C-Motif)ligand 3, Macrophage inflammatory protein 2-beta
Detection Method:Sandwich
Range:7.8-500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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