Human Growth Regulated Oncogene Beta (GROb) ELISA Kit

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SKU:
HUDL01264
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19875
Sensitivity:
6.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL2,SCYB2,MIP2,GRO2,MIP2a,MGSAb,CINC2a,HSF,Macrophage inflammatory protein 2-alpha,Hematopoietic synergistic factor,Chemokine(C-X-C-Motif)ligand 2
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Human Growth Regulated Oncogene Beta (GROb) ELISA Kit

The Human Growth Regulated Oncogene Beta (GROβ) ELISA Kit is a powerful tool for detecting and quantifying GROβ levels in human biological samples. This kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results in various research applications.GROβ, also known as CXCL2, is a chemokine that plays a critical role in inflammation and cancer progression. Elevated levels of GROβ have been associated with various diseases, including cancer, inflammatory disorders, and autoimmune conditions.

Studying GROβ levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its user-friendly design and high-performance capabilities, the Human Growth Regulated Oncogene Beta ELISA Kit is an indispensable tool for researchers investigating the role of GROβ in health and disease. Trust AssayGenie for reliable and robust ELISA kits for your research needs.

Product Name:Human Growth Regulated Oncogene Beta (GROb) ELISA Kit
Product Code:HUDL01264
Size:96 Assays
Target:GROb
Synonyms:CXCL2, SCYB2, MIP2, GRO2, MIP2a, MGSAb, CINC2a, HSF, Macrophage inflammatory protein 2-alpha, Hematopoietic synergistic factor, Chemokine(C-X-C-Motif)ligand 2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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