Human Growth Associated Protein 43 (GAP43) ELISA Kit
SKU:
HUDL01151
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P17677
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
F1,B-50,PP46,Neuron Growth-Associated Protein 43,Neuromodulin,Axonal Membrane Protein GAP-43,Protein F1,Calmodulin-Binding Protein P-57,Neural Phosphoprotein B-50
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Growth Associated Protein 43 (GAP43) ELISA Kit
The Human Growth-Associated Protein 43 (GAP43) ELISA Kit is a powerful tool for detecting and quantifying GAP43 levels in biological samples. This kit is specifically designed for use with human serum, plasma, and cell culture supernatants, providing researchers with accurate and reliable results.GAP43 is a critical protein that is associated with neuronal growth and regeneration. It plays a key role in neural development, synaptic plasticity, and nerve regeneration, making it an important biomarker for studying various neurological conditions and disorders.
With its high sensitivity and specificity, the GAP43 ELISA Kit offers researchers a reliable method for measuring GAP43 levels in biological samples, allowing for detailed investigation into the role of this protein in neuronal growth and development. This kit is an essential tool for researchers working in the fields of neuroscience, neurology, and neurodegenerative diseases.
Product Name:
Human Growth Associated Protein 43 (GAP43) ELISA Kit
Product Code:
HUDL01151
Size:
96 Assays
Target:
GAP43
Synonyms:
F1, B-50, PP46, Neuron Growth-Associated Protein 43, Neuromodulin, Axonal Membrane Protein GAP-43, Protein F1, Calmodulin-Binding Protein P-57, Neural Phosphoprotein B-50
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
