The Human GRN (Granulin) CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of granulin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it ideal for a variety of research applications.Granulin is a key protein that plays a role in processes such as inflammation, wound healing, and cell growth. It is also implicated in various diseases including cancer, neurodegenerative disorders, and inflammatory conditions.
By measuring granulin levels, researchers can gain valuable insights into these diseases and potentially develop new therapeutic strategies.Overall, the Human GRN CLIA Kit is a valuable tool for researchers studying the role of granulin in health and disease, offering accurate and precise results for a wide range of applications.
Product Name:
Human GRN (Granulin) CLIA Kit
SKU:
HUES00920
Target:
Human GRN (Granulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GRN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GRN. You can calculate the concentration of Human GRN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-113
100-118
101-113
Average (%)
103
107
108
1:4
Range (%)
93-104
96-110
86-100
Average (%)
98
103
92
1:8
Range (%)
99-111
97-113
92-104
Average (%)
104
105
99
1:16
Range (%)
88-100
87-100
101-118
Average (%)
93
93
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
101-119
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.0
1.86
7.07
0.94
1.81
6.44
Standard deviation
0.12
0.17
0.81
0.09
0.17
0.69
C V (%)
12.0
9.14
11.46
9.57
9.39
10.71
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GRN in samples. No significant cross-reactivity or interference between Human GRN and analogues was observed.
