Human GRIN1 (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1) ELISA Kit
The Human GRIN1 (Glutamate Receptor, Ionotropic, N-methyl D-aspartate 1) ELISA Kit is specifically designed for the precise measurement of GRIN1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.GRIN1 is a key component of the NMDA receptor complex, playing a crucial role in neurotransmission and synaptic plasticity.
Dysregulation of GRIN1 has been linked to various neurological disorders, including schizophrenia, epilepsy, and Alzheimer's disease. Therefore, measuring GRIN1 levels can provide valuable insights into the pathophysiology of these conditions and aid in the development of targeted therapies.Overall, the Human GRIN1 ELISA Kit offers a reliable tool for researchers to investigate the role of GRIN1 in neurological disorders and explore potential therapeutic strategies.
Product Name:
Human GRIN1 (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1) ELISA Kit
SKU:
HUES02634
Target:
Human GRIN1 (Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GRIN1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRIN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRIN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GRIN1. You can calculate the concentration of Human GRIN1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
95-110
91-104
Average (%)
97
100
96
1:4
Range (%)
89-100
81-92
84-97
Average (%)
95
87
90
1:8
Range (%)
88-103
85-98
83-95
Average (%)
94
92
88
1:16
Range (%)
87-103
83-93
86-96
Average (%)
94
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.57
4.77
0.43
1.51
4.4
Standard deviation
0.02
0.07
0.25
0.03
0.06
0.21
C V (%)
4.26
4.46
5.24
6.98
3.97
4.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GRIN1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GRIN1 in samples. No significant cross-reactivity or interference between Human GRIN1 and analogues was observed.
