Human GPT Recombinant Protein (RPPB1827)
- SKU:
- RPPB1827
- Product type:
- Recombinant Protein
- Size:
- 3U
- Species:
- Human
- Target:
- GPT
- Synonyms:
- Alanine aminotransferase 1
- ALT1
- EC 2612
- Glutamate pyruvate transaminase 1
- Source:
- Escherichia Coli
- Uniprot:
- P24298
Description
| Product Name: | Human GPT Recombinant Protein |
| Product Code: | RPPB1827 |
| Size: | 3U |
| Species: | Human |
| Target: | GPT |
| Synonyms: | Alanine aminotransferase 1, ALT1, EC 2.6.1.2, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile liquid formulation. |
| Formulation: | The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate. |
| Stability: | AAT1 although stable at 10°C for 5 days, should be stored below -18°C.Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Biological Activity: | The specific activity was found to be 1,020 U/mg. |
Alanine transaminase or ALT is a transaminaseenzyme.ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamatealanine+ a-ketoglutarate= pyruvate+ glutamateIt is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT). Diagnostically, it is almost always measured in units/litre (U/L).
Alanine Aminotransferase Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT.The ALT is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. |
| UniProt Protein Details: | Protein type:EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: cytosol Molecular Function:alanine transaminase activity Biological Process: amino acid biosynthetic process |
| NCBI Summary: | This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009] |
| UniProt Code: | P24298 |
| NCBI GenInfo Identifier: | 46577683 |
| NCBI Gene ID: | 2875 |
| NCBI Accession: | P24298.3 |
| UniProt Secondary Accession: | P24298,P78398, Q93076, B0YJ18, D3DWM7, |
| UniProt Related Accession: | P24298 |
| Molecular Weight: | 54,637 Da |
| NCBI Full Name: | Alanine aminotransferase 1 |
| NCBI Synonym Full Names: | glutamic--pyruvic transaminase |
| NCBI Official Symbol: | GPT |
| NCBI Official Synonym Symbols: | AAT1; ALT1; GPT1 |
| NCBI Protein Information: | alanine aminotransferase 1 |
| UniProt Protein Name: | Alanine aminotransferase 1 |
| UniProt Synonym Protein Names: | Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1 |
| Protein Family: | Alanine aminotransferase |
| UniProt Gene Name: | GPT |
| UniProt Entry Name: | ALAT1_HUMAN |
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