Human GPR37 (G Protein Coupled Receptor 37) CLIA Kit
The Human GPR37 (G Protein-Coupled Receptor 37) CLIA Kit is designed for the accurate detection of GPR37 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications. GPR37 is a key receptor involved in the regulation of dopamine signaling in the brain and has been implicated in various neurological disorders such as Parkinson's disease and schizophrenia.
By measuring GPR37 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets. The Human GPR37 CLIA Kit from Assaygenie provides researchers with a powerful tool for studying the role of this important receptor in health and disease. With its high performance and ease of use, this kit is a valuable addition to any laboratory conducting research in the field of neuroscience.
Product Name:
Human GPR37 (G Protein Coupled Receptor 37) CLIA Kit
SKU:
HUES00743
Target:
Human GPR37 (G Protein Coupled Receptor 37)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPR37. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPR37 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPR37, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPR37. You can calculate the concentration of Human GPR37 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-114
96-109
97-113
Average (%)
108
103
105
1:4
Range (%)
99-113
94-108
104-117
Average (%)
106
101
110
1:8
Range (%)
93-109
98-111
95-109
Average (%)
100
103
102
1:16
Range (%)
95-111
88-101
102-114
Average (%)
101
95
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-115
105
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.46
175.74
964.24
102.21
187.64
1060.11
Standard deviation
9.84
15.29
73.67
11.71
20.72
97.32
C V (%)
9.07
8.7
7.64
11.46
11.04
9.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPR37 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPR37 in samples. No significant cross-reactivity or interference between Human GPR37 and analogues was observed.
