Human GPER1 (G Protein Coupled Estrogen Receptor 1) ELISA Kit
The Human GPER1 (G-Protein Coupled Estrogen Receptor 1) ELISA Kit is specifically designed for the accurate measurement of GPER1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees reliable and reproducible results, making it an invaluable tool for various research applications.GPER1 plays a critical role in mediating the effects of estrogen and is involved in numerous physiological processes, including cell growth, migration, and survival.
Dysregulation of GPER1 has been associated with various diseases, such as cancer, cardiovascular disorders, and reproductive issues, making it a promising biomarker for understanding and potentially treating these conditions.By utilizing the Human GPER1 ELISA Kit, researchers can gain valuable insights into the role of GPER1 in health and disease, paving the way for future advances in therapeutic interventions and diagnostic strategies.
Product Name:
Human GPER1 (G Protein Coupled Estrogen Receptor 1) ELISA Kit
SKU:
HUES02240
Target:
Human GPER1 (G Protein Coupled Estrogen Receptor 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GPER1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPER1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPER1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GPER1. You can calculate the concentration of Human GPER1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-96
83-95
85-97
Average (%)
91
90
91
1:4
Range (%)
93-109
85-97
90-104
Average (%)
100
90
95
1:8
Range (%)
90-105
85-98
88-101
Average (%)
96
92
93
1:16
Range (%)
94-105
81-93
87-99
Average (%)
99
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
99
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
85-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.94
2.14
7.31
0.86
2.18
7.34
Standard deviation
0.05
0.12
0.34
0.05
0.09
0.39
C V (%)
5.32
5.61
4.65
5.81
4.13
5.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GPER1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPER1 in samples. No significant cross-reactivity or interference between Human GPER1 and analogues was observed.
