Human GPER1 (G Protein Coupled Estrogen Receptor 1) CLIA Kit
The Human GPER1 (G Protein-Coupled Estrogen Receptor 1) CLIA Kit is specifically designed for the accurate measurement of GPER1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and reliable results for a variety of research purposes.GPER1, also known as GPER or GPR30, is a key G protein-coupled receptor that mediates the effects of estrogen in various physiological processes. It plays a crucial role in several health conditions, including cancer, cardiovascular diseases, and metabolic disorders, making it an important target for research and therapeutic interventions.
By utilizing the Human GPER1 CLIA Kit, researchers can gain valuable insights into the role of GPER1 in different diseases and biological pathways, paving the way for the development of targeted treatment strategies and personalized medicine approaches. Trust in the accuracy and precision of this kit to accelerate your research efforts and advance the field of hormone receptor biology.
Product Name:
Human GPER1 (G Protein Coupled Estrogen Receptor 1) CLIA Kit
SKU:
HUES00671
Target:
Human GPER1 (G Protein Coupled Estrogen Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPER1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPER1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPER1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPER1. You can calculate the concentration of Human GPER1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
92-108
101-114
Average (%)
102
99
107
1:4
Range (%)
92-105
104-116
86-96
Average (%)
98
110
91
1:8
Range (%)
93-109
103-115
86-99
Average (%)
100
109
93
1:16
Range (%)
101-116
89-100
89-101
Average (%)
107
95
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-99
94
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.5
142.42
482.38
51.19
143.59
501.52
Standard deviation
5.04
13.09
48.14
5.14
16.69
30.09
C V (%)
9.98
9.19
9.98
10.04
11.62
6.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPER1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPER1 in samples. No significant cross-reactivity or interference between Human GPER1 and analogues was observed.
