The Human GPC4 (Glypican-4) ELISA Kit is a reliable tool for measuring levels of Glypican-4 in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Glypican-4 is a key protein involved in cellular signaling and development, playing a crucial role in various physiological processes. Dysregulation of Glypican-4 has been implicated in diseases such as cancer, diabetes, and developmental disorders, highlighting its significance as a potential biomarker for disease detection and therapeutic development.
With its advanced technology and superior performance, the Human GPC4 ELISA Kit is a valuable asset for researchers investigating the role of Glypican-4 in health and disease. Get reliable and consistent results with this innovative kit from Assay Genie.
Product Name:
Human GPC4 (Glypican 4) ELISA Kit
SKU:
HUES02703
Target:
Human GPC4 (Glypican 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GPC4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPC4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GPC4. You can calculate the concentration of Human GPC4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
99-114
94-108
Average (%)
95
105
101
1:4
Range (%)
92-108
82-94
83-95
Average (%)
99
88
89
1:8
Range (%)
91-107
82-91
84-98
Average (%)
99
87
91
1:16
Range (%)
88-105
86-97
84-96
Average (%)
96
91
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.52
4.92
0.44
1.38
4.54
Standard deviation
0.03
0.07
0.18
0.02
0.06
0.15
C V (%)
6.25
4.61
3.66
4.55
4.35
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GPC4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPC4 in samples. No significant cross-reactivity or interference between Human GPC4 and analogues was observed.
