Human GP4/CD36 (Platelet Membrane Glycoprotein IV) CLIA Kit
The Human GP4 (CD36) Platelet Membrane Glycoprotein IV CLIA Kit is a highly sensitive and specific assay designed for the accurate measurement of GP4 (CD36) levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.GP4 (CD36) is a crucial glycoprotein involved in platelet adhesion and aggregation, playing a key role in thrombosis and cardiovascular diseases. It is also implicated in immune response modulation and lipid metabolism.
The measurement of GP4 (CD36) levels can provide valuable insights into various disease processes, making it a valuable tool for researchers studying cardiovascular diseases, immune disorders, and lipid metabolism.Overall, the Human GP4 (CD36) Platelet Membrane Glycoprotein IV CLIA Kit is a valuable research tool for the accurate quantification of GP4 (CD36) levels, aiding in the study of various physiological and pathological processes.
Product Name:
Human GP4/CD36 (Platelet Membrane Glycoprotein IV) CLIA Kit
SKU:
HUES00613
Target:
Human GP4/CD36 (Platelet Membrane Glycoprotein IV)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GP4/CD36. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GP4/CD36 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GP4/CD36, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GP4/CD36. You can calculate the concentration of Human GP4/CD36 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
100-115
95-108
Average (%)
96
108
101
1:4
Range (%)
99-115
89-101
99-115
Average (%)
105
95
107
1:8
Range (%)
102-116
96-110
98-114
Average (%)
108
102
105
1:16
Range (%)
88-100
103-115
90-104
Average (%)
93
109
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
91-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.52
221.67
819.66
92.55
236.73
831.41
Standard deviation
11.51
23.81
68.2
9.23
20.83
91.54
C V (%)
11.45
10.74
8.32
9.97
8.8
11.01
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GP4/CD36 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GP4/CD36 in samples. No significant cross-reactivity or interference between Human GP4/CD36 and analogues was observed.
