The Human GP-73 (Golgi Protein 73) ELISA Kit is specifically designed for the precise measurement of GP-73 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and reproducible results, making it an invaluable tool for various research applications.GP-73 is a vital protein found in the Golgi apparatus, playing a crucial role in liver disease, particularly in the diagnosis and prognosis of liver fibrosis and cirrhosis. Elevated levels of GP-73 have been linked to liver damage and inflammation, making it a valuable biomarker for monitoring liver health and assessing disease progression.
By using the Human GP-73 ELISA Kit, researchers can gain valuable insights into liver function and disease, paving the way for the development of new diagnostic and therapeutic strategies for liver-related disorders. Trust in the reliability and precision of this ELISA kit to advance your research and broaden your understanding of liver biology.
Product Name:
Human GP-73 (Golgi Protein 73) ELISA Kit
SKU:
HUES02366
Target:
Human GP-73 (Golgi Protein 73)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GP-73. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GP-73 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GP-73, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GP-73. You can calculate the concentration of Human GP-73 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-102
87-99
89-105
Average (%)
97
92
96
1:4
Range (%)
88-100
85-97
84-97
Average (%)
95
91
89
1:8
Range (%)
89-101
79-92
85-99
Average (%)
95
85
91
1:16
Range (%)
89-103
81-91
87-99
Average (%)
96
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.12
4.13
17.51
1.97
4.13
17.08
Standard deviation
0.11
0.21
0.86
0.1
0.22
0.89
C V (%)
5.19
5.08
4.91
5.08
5.33
5.21
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GP-73 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GP-73 in samples. No significant cross-reactivity or interference between Human GP-73 and analogues was observed.
