The Human GNLY (Granulysin) ELISA Kit is specifically designed for the precise measurement of granulysin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it an excellent choice for various research purposes.Granulysin is a key protein involved in immune responses, particularly in the elimination of pathogens and cancer cells.
Its role in the immune system makes it a valuable biomarker for studying infectious diseases, autoimmune disorders, and cancer progression. The Human GNLY ELISA Kit provides researchers with a reliable tool for investigating the role of granulysin in various health conditions and potential therapeutic targets.
Product Name:
Human GNLY (Granulysin) ELISA Kit
SKU:
HUES02622
Target:
Human GNLY (Granulysin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.14 ng/mL
Detection range:
0.23-15 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GNLY. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GNLY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GNLY, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GNLY. You can calculate the concentration of Human GNLY in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
93-107
89-103
Average (%)
95
101
96
1:4
Range (%)
89-103
85-96
82-93
Average (%)
97
90
88
1:8
Range (%)
93-107
88-99
87-101
Average (%)
99
93
92
1:16
Range (%)
90-102
84-98
83-98
Average (%)
97
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
99
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.72
2.13
6.13
0.67
2.27
5.96
Standard deviation
0.04
0.11
0.19
0.03
0.12
0.32
C V (%)
5.56
5.16
3.1
4.48
5.29
5.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GNLY concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GNLY in samples. No significant cross-reactivity or interference between Human GNLY and analogues was observed.
