The Human GLUT3 (Glucose Transporter 3) ELISA Kit is a reliable tool for measuring GLUT3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.GLUT3 is a key glucose transporter protein that plays a crucial role in glucose uptake and metabolism. Dysregulation of GLUT3 has been implicated in various metabolic disorders, including diabetes and cancer.
By detecting and quantifying GLUT3 levels, researchers can gain valuable insights into glucose metabolism and its implications in disease progression and treatment strategies.Overall, the Human GLUT3 ELISA Kit offers a valuable resource for researchers studying glucose transport mechanisms and their impact on health and disease. Its robust performance and ease of use make it an essential tool for advancing our understanding of glucose metabolism and its role in physiological and pathological conditions.
Product Name:
Human GLUT3 (Glucose Transporter 3) ELISA Kit
SKU:
HUES02782
Target:
Human GLUT3 (Glucose Transporter 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GLUT3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLUT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLUT3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GLUT3. You can calculate the concentration of Human GLUT3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
97-114
91-108
Average (%)
91
104
99
1:4
Range (%)
94-107
86-99
88-101
Average (%)
100
92
93
1:8
Range (%)
90-104
83-96
83-98
Average (%)
96
88
90
1:16
Range (%)
89-100
81-94
82-96
Average (%)
94
87
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
89-101
96
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
0.85
3.73
0.6
0.86
3.69
Standard deviation
0.03
0.05
0.16
0.03
0.05
0.18
C V (%)
5.45
5.88
4.29
5.0
5.81
4.88
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GLUT3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLUT3 in samples. No significant cross-reactivity or interference between Human GLUT3 and analogues was observed.
