SLC2A1,SLC2-A1,GLUT,Solute Carrier Family 2 Member 1,Facilitated Glucose Transporter,Glucose transporter type 1, erythrocyte/brain,HepG2 glucose transporter
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Glucose Transporter 1 (GLUT1) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of GLUT1 levels in human samples including serum, plasma, and cell culture supernatants. GLUT1 plays a critical role in glucose transport across cell membranes and is essential for maintaining glucose homeostasis. Dysregulation of GLUT1 has been implicated in various diseases such as diabetes, cancer, and neurological disorders, making it a valuable biomarker for research and potential therapeutic development.
With its reliable and reproducible results, the GLUT1 ELISA Kit is perfect for investigating the role of GLUT1 in various physiological and pathological conditions. Get your hands on this cutting-edge kit today and unlock new insights into glucose metabolism and its implications in human health.
Product Name:
Human Glucose Transporter 1 (GLUT1) ELISA Kit
Product Code:
HUDL01206
Size:
96 Assays
Target:
GLUT1
Synonyms:
SLC2A1, SLC2-A1, GLUT, Solute Carrier Family 2 Member 1,Facilitated Glucose Transporter, Glucose transporter type 1, erythrocyte/brain, HepG2 glucose transporter
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
