Human Glucose 6 Phosphate Dehydrogenase (G6PD) ELISA Kit
The Human G6PD (Glucose-6-Phosphate Dehydrogenase) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of G6PD levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an excellent choice for a variety of research applications.G6PD is a critical enzyme involved in the pentose phosphate pathway, playing a key role in protecting cells from oxidative damage and maintaining redox balance. Deficiencies in G6PD are associated with various health conditions, including hemolytic anemia and favism.
As such, measuring G6PD levels can provide valuable insights into the pathophysiology of these disorders and aid in the development of targeted therapies.Overall, the Human G6PD ELISA Kit is an essential tool for researchers studying G6PD function and its implications in various diseases. With its high performance and user-friendly design, this kit is sure to meet the needs of researchers in the field.
Product Name:
Human Glucose 6 Phosphate Dehydrogenase (G6PD) ELISA Kit
Product Code:
HUDL01131
Size:
96 Assays
Target:
G6PD
Synonyms:
Glucose-6-phosphate 1-dehydrogenase
Detection Method:
Sandwich
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
