The Human GP130 ELISA Kit from AssayGenie is a cutting-edge tool for the precise measurement of GP130 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, providing accurate and reproducible results for a variety of research purposes.GP130 is a vital protein involved in various cellular processes, including cell growth, differentiation, and immune response regulation. Dysregulation of GP130 has been implicated in numerous diseases such as cancer, inflammatory disorders, and autoimmune conditions, highlighting its significance as a biomarker for disease diagnostics and therapeutic development.
With the AssayGenie Human GP130 ELISA Kit, researchers can gain valuable insights into the role of GP130 in health and disease, paving the way for innovative treatments and advancements in the field of biomedical research.
Product Name:
Human Glucoprotein 130 (gp130) ELISA Kit
Product Code:
HUDL01223
Size:
96 Assays
Target:
gp130
Synonyms:
CDw130, CD130, IL6ST, GP130-RAPS, IR6RB, IL6R-Beta, IR6RB, IR6-RB, Interleukin 6 Signal Transducer, Oncostatin M Receptor, Interleukin-6 Receptor Subunit Beta
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
