The Human GLP-2 (Glucagon-Like Peptide 2) ELISA Kit is specifically designed for the accurate quantification of GLP-2 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a diverse range of research applications.GLP-2 is a vital peptide hormone known for its role in regulating intestinal growth and nutrient absorption. It plays a crucial part in maintaining gastrointestinal health and function, making it a key biomarker for studying digestive disorders, inflammatory bowel disease, and other related conditions.
By accurately measuring GLP-2 levels, researchers can gain valuable insights into underlying mechanisms and potential therapeutic strategies.With its user-friendly protocol and reliable performance, the Human GLP-2 ELISA Kit from Assay Genie is an essential tool for investigating the intricate pathways of gastrointestinal physiology and exploring novel treatment options for gastrointestinal diseases. Trust in the accuracy and precision of this ELISA kit to advance your research and uncover new discoveries in the field of gastroenterology.
Product Name:
Human GLP-2 (Glucagon Like Peptide 2) ELISA Kit
SKU:
HUES03124
Target:
Human GLP-2 (Glucagon Like Peptide 2)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GLP-2. During the reaction, GLP-2 in the sample or standard competes with a fixed amount of GLP-2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GLP-2. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GLP-2 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
97-114
89-102
Average (%)
101
105
94
1:4
Range (%)
83-94
91-106
97-112
Average (%)
89
97
103
1:8
Range (%)
91-101
87-101
99-114
Average (%)
96
93
105
1:16
Range (%)
83-96
85-99
97-114
Average (%)
90
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
90-105
97
Cell culture media (n=5)
94-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.2
0.5
1.0
4.3
Standard deviation
0.03
0.05
0.21
0.03
0.04
0.2
C V (%)
6
4.55
5.0
6.0
4.0
4.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of GLP-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes GLP-2 in samples. No significant cross-reactivity or interference between GLP-2 and analogues was observed.
