The Human GKN2 (Gastrokine-2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GKN2 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Gastrokine-2 is a protein that plays a critical role in maintaining gastric mucosal integrity and protecting against inflammation and tissue damage in the stomach.
Dysregulation of GKN2 has been associated with gastric cancer and other gastrointestinal disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human GKN2 CLIA Kit from Assay Genie offers researchers a powerful tool for studying the role of Gastrokine-2 in human health and disease, with high sensitivity and specificity to ensure accurate and reliable results.
Product Name:
Human GKN2 (Gastrokine 2) CLIA Kit
SKU:
HUES01098
Target:
Human GKN2 (Gastrokine 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GKN2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GKN2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GKN2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GKN2. You can calculate the concentration of Human GKN2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
102-119
87-97
Average (%)
106
108
92
1:4
Range (%)
101-116
103-119
104-120
Average (%)
107
109
110
1:8
Range (%)
90-101
86-98
94-109
Average (%)
96
91
100
1:16
Range (%)
88-102
83-96
98-111
Average (%)
95
90
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
95
EDTA plasma (n=5)
98-115
106
Cell culture media (n=5)
99-115
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.39
111.17
368.0
42.61
112.61
357.61
Standard deviation
4.99
13.27
40.81
3.85
9.18
34.47
C V (%)
11.5
11.94
11.09
9.04
8.15
9.64
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GKN2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GKN2 in samples. No significant cross-reactivity or interference between Human GKN2 and analogues was observed.
