Human GHRH (Growth Hormone Releasing Hormone) ELISA Kit
The Human GHRH (Growth Hormone Releasing Hormone) ELISA Kit offered by Assay Genie is designed for the precise measurement of GHRH levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for a variety of research applications.GHRH is a critical hormone that stimulates the release of growth hormone from the pituitary gland, playing a crucial role in growth, metabolism, and overall health.
Dysregulation of GHRH levels has been associated with conditions such as growth disorders, obesity, and metabolic syndrome, making it an important biomarker for studying these conditions and developing potential treatments.With the Human GHRH ELISA Kit from Assay Genie, researchers can confidently analyze GHRH levels in human samples, advancing our understanding of growth hormone regulation and its impact on various physiological processes.
Product Name:
Human GHRH (Growth Hormone Releasing Hormone) ELISA Kit
SKU:
HUES02231
Target:
Human GHRH (Growth Hormone Releasing Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GHRH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GHRH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GHRH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GHRH. You can calculate the concentration of Human GHRH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
88-102
89-100
Average (%)
92
94
95
1:4
Range (%)
93-106
79-94
87-101
Average (%)
99
86
93
1:8
Range (%)
92-104
85-97
82-93
Average (%)
98
91
87
1:16
Range (%)
92-105
78-90
81-96
Average (%)
98
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.24
156.32
414.19
45.98
155.84
451.11
Standard deviation
2.71
9.29
22.24
2.64
8.06
14.57
C V (%)
5.74
5.94
5.37
5.74
5.17
3.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GHRH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GHRH in samples. No significant cross-reactivity or interference between Human GHRH and analogues was observed.
