The Human GHR (Growth Hormone Receptor) ELISA Kit is specifically designed to accurately detect GHR levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing dependable and consistent results for various research applications.The growth hormone receptor is a key component in the regulation of growth and metabolism, influencing processes such as cell growth, cell repair, and metabolism.
Dysregulation of GHR has been linked to conditions such as growth disorders, obesity, and aging-related diseases, making it a critical biomarker for studying these conditions and exploring therapeutic interventions.With the Human GHR ELISA Kit, researchers can confidently measure GHR levels in biological samples, contributing to a better understanding of growth hormone signaling pathways and potential treatment options for related disorders.
Product Name:
Human GHR (Growth Hormone Receptor) ELISA Kit
SKU:
HUES03018
Target:
Human GHR (Growth Hormone Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GHR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GHR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GHR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GHR. You can calculate the concentration of Human GHR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
90-103
96-111
Average (%)
92
95
102
1:4
Range (%)
89-101
87-98
82-92
Average (%)
96
93
87
1:8
Range (%)
87-98
84-95
85-96
Average (%)
93
90
91
1:16
Range (%)
89-102
81-96
87-102
Average (%)
94
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
93-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.4
12.54
38.08
5.76
13.64
38.32
Standard deviation
0.37
0.56
1.94
0.31
0.71
1.36
C V (%)
6.85
4.47
5.09
5.38
5.21
3.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GHR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GHR in samples. No significant cross-reactivity or interference between Human GHR and analogues was observed.
