The Human GH (Growth Hormone) ELISA Kit is a powerful tool for accurately measuring levels of growth hormone in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers reliable and consistent results for a variety of research purposes.Growth hormone plays a vital role in regulating growth, metabolism, and tissue repair in the body. Imbalances in growth hormone levels have been linked to conditions such as dwarfism, acromegaly, and gigantism, making it a critical biomarker for studying these disorders and exploring potential treatment options.
Whether you are conducting basic research or clinical studies, the Human GH ELISA Kit offers a convenient and efficient solution for analyzing growth hormone levels with precision and accuracy. Trust in this kit to enhance your research efforts and unlock new insights into the role of growth hormone in human health and disease.
Product Name:
Human GH (Growth Hormone) ELISA Kit
SKU:
HUES01444
Target:
Human GH (Growth Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GH. You can calculate the concentration of Human GH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
97-113
97-113
Average (%)
94
104
105
1:4
Range (%)
99-114
85-98
94-109
Average (%)
106
92
100
1:8
Range (%)
101-118
79-92
95-106
Average (%)
107
86
100
1:16
Range (%)
98-113
87-101
97-112
Average (%)
106
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
99
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
260.5
733.1
2028.8
238.5
668.3
2208.9
Standard deviation
14.3
40.3
87.2
13.6
35.4
114.9
C V (%)
5.49
5.5
4.3
5.7
5.3
5.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GH in samples. No significant cross-reactivity or interference between Human GH and analogues was observed.
