Glial Fibrillary Acidic Protein (GFAP) is an intermediate filament (IF) protein which belongs to the intermediate filament family. GFAP is expressed in numerous cell types of the central nervous system (CNS), ependymal cells and phosphorylated by PKN1. GFAP, a class-III intermediate filament, is a cell-specific marker during the development of the central nervous system and distinguishes astrocytes from other glial cells. It is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood.
Product Name:
Human GFAP Recombinant Protein (RPES4966)
Product Code:
RPES4966
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Glial Fibrillary Acidic Protein, GFAP
Accession:
P14136
Sequence:
Leu292-Met432
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Glial Fibrillary Acidic Protein is produced by our E.coli expression system and the target gene encoding Leu292-Met432 is expressed with a 6His tag at the N-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
18.7 kDa
AP Mol Mass:
17 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
