Human GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
The Human GDNF (Glial Cell Line-Derived Neurotrophic Factor) ELISA Kit is a powerful tool for detecting GDNF levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it ideal for a variety of research applications.GDNF is a key protein involved in promoting the survival and growth of neurons, particularly dopaminergic neurons in the brain. Its role in neuroprotection and neuroregeneration makes it an important target for studying neurodegenerative diseases such as Parkinson's disease and amyotrophic lateral sclerosis (ALS).
By measuring GDNF levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic strategies.Overall, the Human GDNF ELISA Kit from AssayGenie is a valuable tool for studying the role of GDNF in neurobiology and developing novel treatments for neurological disorders. Order yours today and take your research to the next level.
Product Name:
Human GDNF (Glial Cell Line Derived Neurotrophic Factor) ELISA Kit
SKU:
HUES02517
Target:
Human GDNF (Glial Cell Line Derived Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDNF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDNF. You can calculate the concentration of Human GDNF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
86-96
99-113
Average (%)
103
91
105
1:4
Range (%)
89-106
82-97
83-96
Average (%)
97
88
90
1:8
Range (%)
90-102
81-93
87-102
Average (%)
96
86
93
1:16
Range (%)
88-104
86-98
87-99
Average (%)
95
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
87-99
92
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.18
9.18
1.06
2.09
9.23
Standard deviation
0.06
0.11
0.35
0.07
0.11
0.4
C V (%)
5.66
5.05
3.81
6.6
5.26
4.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDNF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDNF in samples. No significant cross-reactivity or interference between Human GDNF and analogues was observed.
