Human GDF9 (Growth Differentiation Factor 9) ELISA Kit
The Human GDF9 (Growth Differentiation Factor 9) ELISA Kit is specifically designed for the precise measurement of GDF9 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit guarantees accurate and consistent results, making it an invaluable tool for various research applications.GDF9 is a key protein that plays a critical role in the growth and differentiation of cells, particularly in reproductive tissues.
Its involvement in regulating ovarian function and follicular development makes it an important marker for studying fertility issues and reproductive disorders. This ELISA kit offers researchers the opportunity to explore the role of GDF9 in such conditions and potentially discover new therapeutic approaches.
Product Name:
Human GDF9 (Growth Differentiation Factor 9) ELISA Kit
SKU:
HUES02860
Target:
Human GDF9 (Growth Differentiation Factor 9)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDF9. You can calculate the concentration of Human GDF9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
93-105
100-116
Average (%)
95
98
106
1:4
Range (%)
88-102
81-92
85-101
Average (%)
94
87
92
1:8
Range (%)
91-104
79-90
86-99
Average (%)
96
85
93
1:16
Range (%)
93-108
80-95
86-97
Average (%)
99
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
95
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.84
4.97
0.51
0.82
4.85
Standard deviation
0.03
0.04
0.23
0.03
0.04
0.15
C V (%)
6
4.76
4.63
5.88
4.88
3.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDF9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF9 in samples. No significant cross-reactivity or interference between Human GDF9 and analogues was observed.
