Human GDF15 (Growth Differentiation Factor 15) ELISA Kit
The Human GDF15 (Growth Differentiation Factor 15) ELISA Kit is specially designed to accurately measure GDF15 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GDF15 is a key protein involved in regulating cell growth, differentiation, and inflammation. It has been implicated in various diseases including cancer, cardiovascular diseases, and metabolic disorders, making it a valuable biomarker for studying disease progression and identifying potential therapeutic targets.
With its reliable performance and broad applicability, the Human GDF15 ELISA Kit from Assay Genie is an essential tool for researchers seeking to deepen their understanding of GDF15 biology and its role in human health and disease.
Product Name:
Human GDF15 (Growth Differentiation Factor 15) ELISA Kit
SKU:
HUES01367
Target:
Human GDF15 (Growth Differentiation Factor 15)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
14.06 pg/mL
Detection range:
23.44-1500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF15. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF15, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDF15. You can calculate the concentration of Human GDF15 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
84-99
96-113
Average (%)
100
91
103
1:4
Range (%)
91-103
86-98
84-99
Average (%)
96
91
90
1:8
Range (%)
90-106
81-92
82-93
Average (%)
98
87
88
1:16
Range (%)
87-103
84-96
88-102
Average (%)
94
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
87-102
94
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
83.3
126.61
615.36
76.84
120.76
573.31
Standard deviation
4.87
6.79
32.0
4.78
6.96
27.46
C V (%)
5.85
5.36
5.2
6.22
5.76
4.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDF15 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF15 in samples. No significant cross-reactivity or interference between Human GDF15 and analogues was observed.
