Human GDF11 (Growth Differentiation Factor 11) ELISA Kit
The Human GDF11 (Growth Differentiation Factor 11) ELISA Kit is designed for the precise measurement of GDF11 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an ideal tool for various research applications.GDF11 is a key protein that regulates cell growth and differentiation, playing a critical role in developmental processes and tissue regeneration.
Its dysregulation has been linked to various diseases such as cardiovascular disorders, musculoskeletal conditions, and aging-related pathologies, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.For more information or to purchase the Human GDF11 ELISA Kit, please visit www.assaygenie.com/human-gdf11-growth-differentiation-factor-11-elisa-kit-hues02855/.
Product Name:
Human GDF11 (Growth Differentiation Factor 11) ELISA Kit
SKU:
HUES02855
Target:
Human GDF11 (Growth Differentiation Factor 11)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF11. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDF11. You can calculate the concentration of Human GDF11 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
87-99
94-111
Average (%)
103
94
101
1:4
Range (%)
89-105
86-100
89-99
Average (%)
96
91
94
1:8
Range (%)
87-100
81-93
88-103
Average (%)
94
86
94
1:16
Range (%)
90-104
82-93
83-98
Average (%)
97
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
99
EDTA plasma (n=5)
92-103
97
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.84
119.44
417.65
54.28
109.97
441.82
Standard deviation
3.4
5.23
21.26
3.7
5.33
13.7
C V (%)
6.43
4.38
5.09
6.82
4.85
3.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDF11 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF11 in samples. No significant cross-reactivity or interference between Human GDF11 and analogues was observed.
