Human GDF10 (Growth Differentiation Factor 10) ELISA Kit
The Human GDF10 (Growth Differentiation Factor 10) ELISA Kit is specifically designed for the precise measurement of GDF10 levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.GDF10, also known as BMP-3b, is a member of the Bone Morphogenetic Protein (BMP) family and plays a vital role in regulating cell growth, differentiation, and development. It is implicated in processes such as bone and cartilage formation, as well as neurological functions.
Understanding the role of GDF10 is essential in studying conditions like skeletal disorders, cancer, and neurodegenerative diseases.By utilizing the Human GDF10 ELISA Kit, researchers can explore the implications of GDF10 in various biological processes and diseases, facilitating the development of potential therapeutic interventions. This kit provides a reliable and efficient method for quantifying GDF10 levels, contributing to advancements in biomedical research.
Product Name:
Human GDF10 (Growth Differentiation Factor 10) ELISA Kit
SKU:
HUES02854
Target:
Human GDF10 (Growth Differentiation Factor 10)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GDF10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GDF10. You can calculate the concentration of Human GDF10 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
97-111
87-98
Average (%)
94
102
93
1:4
Range (%)
93-107
86-99
84-95
Average (%)
99
92
90
1:8
Range (%)
93-105
78-90
82-98
Average (%)
99
85
89
1:16
Range (%)
89-103
80-95
87-101
Average (%)
95
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
95-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
96.13
263.59
721.1
104.18
266.73
738.96
Standard deviation
5.52
13.13
22.93
5.74
12.64
40.27
C V (%)
5.74
4.98
3.18
5.51
4.74
5.45
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GDF10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GDF10 in samples. No significant cross-reactivity or interference between Human GDF10 and analogues was observed.
