Human GCK Recombinant Protein (RPPB2541)

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SKU:
RPPB2541
Product type:
Recombinant Protein
Size:
5ug
Species:
Human
Target:
GCK
Synonyms:
Glucokinase
Synonyms:
Glucokinase (Hexokinase 4)
Synonyms:
Hexokinase Type IV
Synonyms:
HK IV
Source:
Escherichia Coli
Uniprot:
P35557
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Description

Product Name:Human GCK Recombinant Protein
Product Code:RPPB2541
Size:5µg
Species:Human
Target:GCK
Synonyms:Glucokinase, Glucokinase (Hexokinase 4), Hexokinase Type IV, HK IV, HK4, Maturity Onset Diabetes of The Young 2, ATP:D-Hexose 6-Phosphotransferase, Hexokinase D, Pancreatic Isozyme, Hexokinase-4, Hexokinase-D, Hexokinase 4.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:GCK protein solution (1mg/ml) contains 20 mM Tris-HCl buffer (pH 8.0) and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ
Biological Activity:Specific activity is > 2,000 pmol/min/ug. One unit will convert 1 pmoles of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.

GCK is an enzyme that expedite the formation of glucose-6-phosphate for glucose by phosphorylation. Humans and other vertebrates have GCK in the cells of the pancreas and liver. In both organs, the enzyme has an important role in carbohydrate metabolism regulation by sensing sugar levels and acting according to the change in glucose levels, that can rise after a meal or fall during fasting. Mutations in the gene that codes for this enzyme can cause hypoglycemia or diabetes..

GCK Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 485 amino acids (1-465 a.a) and having a molecular mass of 54.3kDa. GCK is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Code:P35557
NCBI GenInfo Identifier:547696
NCBI Gene ID:2645
NCBI Accession:P35557.1
UniProt Secondary Accession:P35557,Q05810, A4D2J2, A4D2J3,
UniProt Related Accession:P35557
Molecular Weight:465
NCBI Full Name:Glucokinase
NCBI Synonym Full Names:glucokinase (hexokinase 4)
NCBI Official Symbol:GCK  
NCBI Official Synonym Symbols:GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3  
NCBI Protein Information:glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase
UniProt Protein Name:Glucokinase
UniProt Synonym Protein Names:Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D
UniProt Gene Name:GCK  
UniProt Entry Name:HXK4_HUMAN
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