Human GCK Recombinant Protein (RPPB2540)
- SKU:
- RPPB2540
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- GCK
- Synonyms:
- Glucokinase
- EC 2712
- Hexokinase-4
- Hexokinase type IV
- Source:
- Escherichia Coli
- Uniprot:
- P35557
Description
| Product Name: | Human GCK Recombinant Protein |
| Product Code: | RPPB2540 |
| Size: | 10µg |
| Species: | Human |
| Target: | GCK |
| Synonyms: | Glucokinase, EC 2.7.1.2, Hexokinase-4, Hexokinase type IV, HK IV, HK4, Hexokinase-D, GCK, GK, GLK, HHF3, HKIV, HXKP, MODY2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The protein (1mg/ml) contains 20mM Tris-HCl pH-8.0 and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA LVSAVACKKA CMLGQ |
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of Glucokinase results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, HK4 is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Glucokinase Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa 1-465) fused to a 20aa His tag at the N-terminal encoding the sequence of 485 amino acids and having a molecular mass of 54.3 kDa.HK4 is purified by proprietary chromatographic techniques.
| UniProt Code: | P35557 |
| NCBI GenInfo Identifier: | 547696 |
| NCBI Gene ID: | 2645 |
| NCBI Accession: | P35557.1 |
| UniProt Secondary Accession: | P35557,Q05810, A4D2J2, A4D2J3, |
| UniProt Related Accession: | P35557 |
| Molecular Weight: | 465 |
| NCBI Full Name: | Glucokinase |
| NCBI Synonym Full Names: | glucokinase (hexokinase 4) |
| NCBI Official Symbol: | GCK |
| NCBI Official Synonym Symbols: | GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3 |
| NCBI Protein Information: | glucokinase; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; hexokinase D, pancreatic isozyme; ATP:D-hexose 6-phosphotransferase |
| UniProt Protein Name: | Glucokinase |
| UniProt Synonym Protein Names: | Hexokinase type IV; HK IV; Hexokinase-4; HK4; Hexokinase-D |
| UniProt Gene Name: | GCK |
| UniProt Entry Name: | HXK4_HUMAN |
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