Human GAP43 (Growth Associated Protein 43) ELISA Kit
The Human GAP43 (Growth-Associated Protein 43) ELISA Kit is specifically designed for the precise measurement of GAP43 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.GAP43 is a key protein that plays a crucial role in neural growth and regeneration. It is involved in the development and maintenance of the nervous system, making it a valuable biomarker for studying neurological disorders, brain injuries, and neurodegenerative diseases.
With its reliable performance and broad applicability, the Human GAP43 ELISA Kit is an essential tool for researchers studying neural development, regeneration, and diseases related to the nervous system. Get your kit today and advance your research in this important area of study.
Product Name:
Human GAP43 (Growth Associated Protein 43) ELISA Kit
SKU:
HUES02868
Target:
Human GAP43 (Growth Associated Protein 43)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAP43. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAP43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAP43, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAP43. You can calculate the concentration of Human GAP43 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
93-108
84-98
Average (%)
102
100
90
1:4
Range (%)
91-105
82-96
82-94
Average (%)
96
88
89
1:8
Range (%)
87-101
80-90
81-95
Average (%)
93
85
87
1:16
Range (%)
89-101
83-97
88-100
Average (%)
95
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
0.97
4.0
0.49
0.9
4.2
Standard deviation
0.03
0.05
0.17
0.03
0.04
0.13
C V (%)
5.66
5.15
4.25
6.12
4.44
3.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GAP43 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAP43 in samples. No significant cross-reactivity or interference between Human GAP43 and analogues was observed.
