Human Gamma-Aminobutyric Acid A Receptor Alpha 2 (gABRa2) ELISA Kit
The Human Gamma-Aminobutyric Acid A Receptor Alpha 2 (GABRA2) ELISA Kit is a cutting-edge tool for the precise measurement of GABRA2 levels in human biological samples. This advanced kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The GABRA2 receptor is a key player in the regulation of neurotransmission in the central nervous system, particularly in processes involving inhibition and neuronal excitability. Dysregulation of this receptor has been implicated in various neurological disorders, including epilepsy, anxiety, and alcohol dependence, making it a valuable target for research and therapeutic development.
By utilizing the Human GABRA2 ELISA Kit, researchers can gain valuable insights into the role of GABRA2 in neurological function and dysfunction, paving the way for new discoveries and potential treatment strategies. This kit is a must-have for any scientist studying the intricate mechanisms of the brain and seeking to unravel the mysteries of neurological disorders.
Product Name:
Human Gamma-Aminobutyric Acid A Receptor Alpha 2 (gABRa2) ELISA Kit
Product Code:
HUDL01134
Size:
96 Assays
Target:
gABRa2
Synonyms:
GABA(A) receptor subunit alpha-2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
