Human GALE Recombinant Protein (RPES2211)

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SKU:
RPES2211
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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Description

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Human GALE Recombinant Protein

The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.

Product Name:Human GALE Recombinant Protein (RPES2211)
Product Code:RPES2211
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:UDP-Glucose 4-Epimerase, Galactowaldenase, UDP-Galactose 4-Epimerase, GALE
Accession:Q14376
Sequence:Met 1-Ala348
Fusion tag:N-6His
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human UDP-Glucose 4-Epimerase is produced by our E.coli expression system and the target gene encoding Met1-Ala348 is expressed with a 6His tag at the N-terminus.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:40.4 kDa
AP Mol Mass:35 kDa
Formulation:Supplied as a 0.2 µm filtered solution of 50mM TrisHCl, 150mM NaCl, 2mM DTT, 1mM EDTA, pH 8.0.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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