The Human Gal12 (Galectin-12) CLIA Kit is a powerful tool for measuring galectin-12 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research purposes.Galectin-12 is a key protein involved in various biological processes, including inflammation and immune response modulation. Abnormal galectin-12 levels have been associated with conditions such as autoimmune diseases, diabetes, and cancer.
Therefore, studying galectin-12 levels can provide valuable insights into these diseases and potentially lead to the development of new therapeutic interventions.With its ease of use and consistent performance, the Human Gal12 CLIA Kit is an indispensable tool for researchers exploring the role of galectin-12 in health and disease. Order yours today from Assay Genie to accelerate your research progress.
Product Name:
Human GAL12 (Galectin 12 ) CLIA Kit
SKU:
HUES00617
Target:
Human GAL12 (Galectin 12 )
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAL12. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL12, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GAL12. You can calculate the concentration of Human GAL12 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
90-101
99-112
Average (%)
104
95
106
1:4
Range (%)
100-112
93-105
86-97
Average (%)
106
100
92
1:8
Range (%)
102-121
96-110
102-115
Average (%)
110
104
108
1:16
Range (%)
103-116
96-111
93-104
Average (%)
109
103
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
102
EDTA plasma (n=5)
94-109
101
Cell culture media (n=5)
93-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
215.64
395.08
1907.93
198.64
361.15
1921.27
Standard deviation
23.79
41.8
168.85
22.39
27.27
165.61
C V (%)
11.03
10.58
8.85
11.27
7.55
8.62
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GAL12 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL12 in samples. No significant cross-reactivity or interference between Human GAL12 and analogues was observed.
