The Human Gal1 (Galectin-1) ELISA Kit is specifically designed to detect Galectin-1 levels in human serum, plasma, and cell culture supernatants with high sensitivity and specificity. This reliable and reproducible kit is ideal for a variety of research applications.Galectin-1 is a key protein involved in cell adhesion, cell activation, and modulation of immune responses. It plays a crucial role in various physiological and pathological processes, including cancer, inflammation, and autoimmune diseases.
Therefore, this ELISA kit is essential for studying these conditions and developing potential therapies.With easy-to-follow instructions and quick assay procedures, the Human Gal1 (Galectin-1) ELISA Kit from Assaygenie is a valuable tool for researchers looking to accurately measure Galectin-1 levels and further understand its biological functions.
Product Name:
Human GAL1 (Galectin 1 ) ELISA Kit
SKU:
HUES02150
Target:
Human GAL1 (Galectin 1 )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GAL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GAL1. You can calculate the concentration of Human GAL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
92-106
86-99
Average (%)
92
98
92
1:4
Range (%)
87-101
81-92
87-99
Average (%)
93
87
93
1:8
Range (%)
91-106
87-100
88-103
Average (%)
99
92
94
1:16
Range (%)
92-107
81-94
86-97
Average (%)
98
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
100
EDTA plasma (n=5)
91-104
98
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.4
5.79
22.26
2.27
5.27
20.42
Standard deviation
0.14
0.28
1.19
0.14
0.29
0.82
C V (%)
5.83
4.84
5.35
6.17
5.5
4.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GAL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GAL1 in samples. No significant cross-reactivity or interference between Human GAL1 and analogues was observed.
