Human G CSF Recombinant Protein (RPPB0316)
- SKU:
- RPPB0316
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- G CSF
- Synonyms:
- CSF-3
- MGI-1G
- GM-CSF beta
- Pluripoietin
- Source:
- Escherichia Coli
- Uniprot:
- P09919
Description
| Product Name: | Human G CSF Recombinant Protein |
| Product Code: | RPPB0316 |
| Size: | 10µg |
| Species: | Human |
| Target: | G CSF |
| Synonyms: | CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Lenograstim, G-CSF, MGC45931, GCSF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH= 4. |
| Solubility: | It is recommended to reconstitute the lyophilized GCSF in sterile 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized GCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids of GCSF was determined and was found to be Met-Thr-Pro-Leu-Gly |
| Biological Activity: | The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml, corresponding to a Specific Activity of 100,000,000 IU/mg. |
GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes.
Granulocyte Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 KD.GCSF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine; Secreted, signal peptide; Cell cycle regulation; Secreted Chromosomal Location of Human Ortholog: 17q11.2-q12 Cellular Component: extracellular space Molecular Function:enzyme binding; growth factor activity; cytokine activity; granulocyte colony-stimulating factor receptor binding Biological Process: positive regulation of protein binding; positive regulation of myeloid cell differentiation; granulocyte differentiation; multicellular organismal development; cytokine and chemokine mediated signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; immune response; positive regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
| UniProt Code: | P09919 |
| NCBI GenInfo Identifier: | 117564 |
| NCBI Gene ID: | 1440 |
| NCBI Accession: | P09919.1 |
| UniProt Secondary Accession: | P09919,A8MXR7, |
| UniProt Related Accession: | P09919 |
| Molecular Weight: | 18,434 Da |
| NCBI Full Name: | Granulocyte colony-stimulating factor |
| NCBI Synonym Full Names: | colony stimulating factor 3 (granulocyte) |
| NCBI Official Symbol: | CSF3 |
| NCBI Official Synonym Symbols: | GCSF; CSF3OS; C17orf33 |
| NCBI Protein Information: | granulocyte colony-stimulating factor; filgrastim; lenograstim; pluripoietin |
| UniProt Protein Name: | Granulocyte colony-stimulating factor |
| UniProt Synonym Protein Names: | Pluripoietin; INN: Filgrastim; INN: Lenograstim |
| Protein Family: | Granulocyte colony-stimulating factor |
| UniProt Gene Name: | CSF3 |
| UniProt Entry Name: | CSF3_HUMAN |
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