The Human FTH (Ferritin Heavy Polypeptide) ELISA Kit is a powerful tool for the precise measurement of FTH levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research endeavors. FTH, a key component of the ferritin protein complex, plays a crucial role in iron metabolism and storage. Dysregulation of FTH has been implicated in various diseases such as iron overload disorders, inflammatory conditions, and certain cancers, highlighting its significance as a biomarker for disease diagnosis and management.
Whether investigating the role of FTH in disease pathogenesis or exploring its therapeutic potential, the Human FTH ELISA Kit offers researchers a reliable and efficient solution for their experimental needs. Trust in this kit to deliver precise and reliable data, advancing your understanding of FTH biology and its implications in human health and disease.
Product Name:
Human FTH (Ferritin, Heavy Polypeptide) ELISA Kit
SKU:
HUES02940
Target:
Human FTH (Ferritin, Heavy Polypeptide)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
7.50 ng/mL
Detection range:
12.50-800 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FTH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FTH. You can calculate the concentration of Human FTH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
90-105
99-114
Average (%)
92
96
104
1:4
Range (%)
88-100
84-99
88-101
Average (%)
93
91
93
1:8
Range (%)
91-107
83-96
83-95
Average (%)
97
88
89
1:16
Range (%)
93-104
85-97
84-97
Average (%)
98
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
90
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
88-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
38.61
98.0
378.23
40.84
105.88
387.76
Standard deviation
2.23
5.75
12.71
2.72
6.11
15.16
C V (%)
5.78
5.87
3.36
6.66
5.77
3.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FTH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FTH in samples. No significant cross-reactivity or interference between Human FTH and analogues was observed.
