The Human FST (Follistatin) ELISA Kit is specifically designed to detect levels of follistatin in human samples such as serum, plasma, and cell culture supernatants with high sensitivity and specificity. Follistatin is a critical protein known for its role in regulating activin, a growth and differentiation factor, making it a key player in various cellular processes.This kit provides accurate and reproducible results, making it an excellent tool for researchers studying follistatin's involvement in conditions such as muscle growth, reproduction, and inflammation.
Its versatility allows for a wide range of research applications, making it an essential component in understanding the mechanisms behind various physiological and pathological processes.By utilizing the Human FST (Follistatin) ELISA Kit, researchers can gain valuable insights into the functions of follistatin and its potential implications in various diseases, paving the way for new discoveries and therapeutic interventions.
Product Name:
Human FST (Follistatin) ELISA Kit
SKU:
HUES01363
Target:
Human FST (Follistatin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.15 ng/mL
Detection range:
0.25-16 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human FST. You can calculate the concentration of Human FST in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
93-104
97-114
Average (%)
101
99
104
1:4
Range (%)
92-104
83-97
88-99
Average (%)
97
90
94
1:8
Range (%)
91-102
80-93
80-95
Average (%)
97
86
87
1:16
Range (%)
90-102
83-96
83-96
Average (%)
97
89
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.79
1.71
6.71
0.86
1.67
6.11
Standard deviation
0.04
0.07
0.3
0.05
0.08
0.23
C V (%)
5.06
4.09
4.47
5.81
4.79
3.76
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human FST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FST in samples. No significant cross-reactivity or interference between Human FST and analogues was observed.
