The Human FPB (Fibrinopeptide B) CLIA Kit is a reliable and sensitive assay designed for the precise quantification of fibrinopeptide B levels in human samples, including serum and plasma. The kit offers high specificity and accuracy, ensuring consistent and reproducible results that are suitable for a wide range of research applications.Fibrinopeptide B is a key component in the blood clotting process, playing a crucial role in the formation of fibrin, a protein essential for blood clotting. Abnormal levels of fibrinopeptide B have been associated with various conditions, including thrombosis, cardiovascular diseases, and inflammatory disorders, making it a valuable biomarker for studying and understanding these diseases.
The Human FPB CLIA Kit provides researchers with a powerful tool for investigating the role of fibrinopeptide B in disease pathology and developing potential therapeutic interventions. Its high sensitivity and accuracy make it an invaluable asset for advancing research in the field of coagulation and thrombosis.
Product Name:
Human FPB (Fibrinopeptide B) CLIA Kit
SKU:
HUES01158
Target:
Human FPB (Fibrinopeptide B)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FPB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FPB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FPB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FPB. You can calculate the concentration of Human FPB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
92-105
90-106
Average (%)
96
99
97
1:4
Range (%)
97-111
92-104
99-115
Average (%)
104
99
107
1:8
Range (%)
92-109
89-105
87-99
Average (%)
100
96
94
1:16
Range (%)
91-105
101-114
102-117
Average (%)
98
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
96
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
96-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.89
121.8
467.34
51.73
117.74
449.6
Standard deviation
4.01
14.46
50.33
4.91
12.01
29.63
C V (%)
8.2
11.87
10.77
9.49
10.2
6.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FPB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FPB in samples. No significant cross-reactivity or interference between Human FPB and analogues was observed.
