The Human FOXC2 (Forkhead Box Protein C2) CLIA Kit is a highly accurate assay designed for the quantitative detection of FOXC2 levels in human samples such as serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit provides dependable and consistent results, making it an ideal tool for various research applications.FOXC2 is a critical transcription factor that plays a key role in regulating gene expression related to embryonic development and cell differentiation. Dysregulation of FOXC2 has been implicated in various diseases including cancer, cardiovascular disorders, and metabolic syndromes, highlighting its importance as a potential biomarker for disease diagnosis and prognosis.
With the Human FOXC2 CLIA Kit, researchers can accurately measure FOXC2 levels in biological samples, enabling in-depth studies on its physiological functions and pathological implications. This kit is a valuable resource for investigating the role of FOXC2 in disease mechanisms and developing targeted therapeutic strategies.
Product Name:
Human FOXC2 (Forkhead Box Protein C2) CLIA Kit
SKU:
HUES00883
Target:
Human FOXC2 (Forkhead Box Protein C2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FOXC2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FOXC2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FOXC2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human FOXC2. You can calculate the concentration of Human FOXC2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
86-99
86-100
Average (%)
98
93
92
1:4
Range (%)
90-105
85-99
97-113
Average (%)
97
91
104
1:8
Range (%)
93-109
97-109
99-115
Average (%)
100
103
107
1:16
Range (%)
90-101
96-110
96-110
Average (%)
96
102
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
96
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.62
180.71
702.44
107.9
169.64
723.87
Standard deviation
11.82
18.69
58.93
12.98
17.13
70.65
C V (%)
11.75
10.34
8.39
12.03
10.1
9.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human FOXC2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human FOXC2 in samples. No significant cross-reactivity or interference between Human FOXC2 and analogues was observed.
